<progress id="3ty9u"><track id="3ty9u"></track></progress>

<rp id="3ty9u"><ruby id="3ty9u"><blockquote id="3ty9u"></blockquote></ruby></rp>

<span id="3ty9u"></span>
    <tbody id="3ty9u"></tbody>

          Reagent Center

          Spin Column Method

          • Biospin Total RNA Extraction Kit II MORE
            Product Details:

            This kit is a column-type total RNA extraction kit based on the improved lysis and binding reagent components, which improves the lysis and binding performance of the reagent. The addition of chloroform realizes the separation of impurities such as RNA from DNA and protein, and optimizes the column membrane to improve its ability to adsorb RNA. After multiple washings, impurities are effectively removed to the greatest extent, and high-purity and high-quality total RNA is obtained. Nucleic acid can be extracted from a wide range of samples such as blood, animal and plant tissues, various microorganisms and cultured cells. High-quality nucleic acid products can be directly used in downstream molecular experiments such as Northern Blot, RT-qPCR, and NGS.

            Product Features:

            1.  High purity

            2.  No DNA residue

            3.  Sufficient protein removal

            4.  Simple operation

            Experimental data:

            Case 1 

            1. Extract 1mL (106/mL) of monolayer cultured cells, 100μL of Elution buffer, use Nanodrop 2000 to measure the concentration and 1% agarose gel electrophoresis to detect the integrity of nucleic acid bands, and compare with similar brands reagents.

            Reagent brand

            Concentration (ng/μL)

            260/280

            260/230

            Bioer company

            361.4

            2.06

            1.82

            T company

            352

            2.08

            1.9

            N company

            356.3

            2.06

            1.72

            Case 2

            Extract 50mg of the leaf tissues of  Buxus microphylla and Ophiopogon japonicus, 100μL Elution Buffer, use Nanodrop 2000 to measure the concentration and 1% agarose gel electrophoresis to detect the integrity of nucleic acid bands, and compare with similar brands reagents.

            Sample

            T company

            Bioer company

            Concentration (ng/μL)

            260/280

            260/230

            Concentration (ng/μL)

            260/280

            260/230

            Buxus microphylla

            204.3

            2

            1.03

            228.9

            2.12

            1.6

            Ophiopogon japonicus 

            251.8

            2.13

            2.15

            301.5

            2.12

            1.26

             

            The results show that the extraction efficiency of Bioer's kit is better than that of similar products of other brands.

             

            Manual download
          • Biospin Virus DNA Extraction Kit II MORE
            Product Details:

            This kit uses a unique lysis solution to lyse the virus, and is suitable for extracting viral DNA from ≤200μL plasma, serum, cell-free body fluid, and cultured animal cell supernatants. The purified viral DNA can be widely used in Real-time PCR, Sequencing, Southern Blot, Mutant Analysis, SNP and other common downstream experiments in molecular biology.

             

            Product Features:
            1. Fast and efficient: the purification time of virus DNA is less than 30 minutes;
            2. Easy to operate: no need for Carrier RNA;
            3. Safe and non-toxic: safe and environmentally friendly, without irritating and toxic ingredients;
            4. High purity: impurities are removed cleanly and can be directly applied to downstream analysis and detection
            Experimental data:

            Case 1

            Extract DNA (5~105IU/mL) from serially diluted serum samples of hepatitis B virus (HBV) positive serum, and perform high-sensitivity HBV fluorescence quantitative PCR detection. The results are as follows:

             

            Samples(IU/ml)  

            CT Value

            E5

            22.69

            E4

            25.34

            E3

            28.71

            E2

            31.3

            25

            33.38

            10

            33.88

            5

            36.09

            Negative control

            -

             

            Case 2 

            A customer used the BIOER Biospin Virus DNA Extraction Kit II to compare with similar products from S company, extract 4 environmental samples, and perform fluorescence quantitative PCR amplification at the same time to detect the presence of African swine fever virus in the samples. The results are as follows:

                       Ct value

            Sample

            Bioer

            Ct value

            S Company

            Ct value

            1

            27.61

            29.07

            2

            28.26

            30.94

            3

            No Ct

            No Ct

            4

            No Ct

            No Ct

            The above results show that the BIOER Biospin virus DNA extraction kit II product is superior to the similar products of S company.

            Manual download
          • SimplyP Animal pathogens DNA/RNA Extraction Kit MORE
            Product Details:

            This product provides a set of methods for quickly extracting DNA and RNA viruses from tissue homogenate supernatant and serum, plasma, whole blood and ascites samples. The extracted nucleic acid can be directly used for PCR, RT-PCR/Real-time PCR Wait for downstream experiments.

             

            Product Features:

            1、The sample can be extracted within 11 minutes.

            2、Less steps: 4 steps can be completed.

            3、New technology: high sensitivity can be achieved without using reagents such as proteinase K and Carrier RNA.

            4、Wide range of applications: suitable for extracting various DNA and RNA viruses

            Experimental data:

            Case 1 

            Extract cat feces samples for real-time PCR amplification of cat coronavirus:

            Result

            Sample

            Ct Value

            1

            17.79

            2

            21.4

            3

            25.18

            4

            25.28

            5

            29.29

            6

            33.66

            7

            NoCt

             

             

             

             

             

            1:Original template ; 2:10-1; 3:Positive control;4:10-2;5;10-3;6:10-4;7:Negative control

             

            Case 2 

            Extract cat snout and nose swab samples for real-time PCR amplification of feline herpes virus: 

            Result

            Sample

            Ct Value

            Original liquid

            20.26

            20.08

            10 times dilution

            23.42

            23.57

            100 times dilution

            27.09

            26.93

            1000 times dilution

            30.45

            30.55

            10000 times dilution

            34.06

            34.17

            Negative control

            NoCt

            NoCt

             

             

             

             

             

            Manual download
          • Biospin Virus DNA/RNA Extraction Kit MORE
            Product Details:

            The Biospin Virus DNA/RNA Extraction Kit is designed for the rapid and efficient isolation and purification of high quality virus nucleic acid from a variety of samples. It takes about only 30 minutes for the total procedure.
            The obtained virus nucleic acid can be used directly for a broad range of downstream applications, such as qPCR, NGS, etc




            Features Specifications
            Format
            Spin Column
            Technology Silica membrane technology
            Sample Serum, Plasma, Whole blood, Swabs, Saliva,
            Body fluid, Tissue, Feces, BALF, etc.
            Sample Volume 200 μL
            Elution Volume 50-100 μL
            Processing Manual (Centrifugation)
            Processing Time 30 minutes
            Yield High purity viral DNA or Viral RNA
            Storage Conditions 2-30°C (Protease K: 2-8°C)
            Shelf life 12 months


            Product Features:

            1.Rapid and Reliable: Fast procedures and easy to use
            2.High Sensitivity: DNA: 10 IU/mL, RNA: 50 IU/mL
            3.Versatile: Nucleic acid of various virus, e.g. SARS-CoV-2, Hepatitis, etc. in different samples, such as Serum, Plasma, Whole blood, Swabs, Saliva,Body fluid, Tissue, Feces, BALF, etc. for a broad range of down stream applications
            4.Safe: Toxic free

            Experimental data:

            Case 1 To Extract RNA from Coronavirus

            Gradient dilute the supernatant of fecal sample which contain Coronavirus, using the original and the diluted solution as samples, extract the Coronavirus RNA of each samples by using Biospin Virus DNA/RNA Extraction Kit and a leading brand extraction kit from Q company separately, then detect the Coronavirus RNA concentration by Real-Time RT-PCR. The results are as follows:

            Case 4 To Extract DNA from African Swine Fever Virus

            Gradient dilute the tissue homogenate that contains African Swine Fever Virus, using the original and the diluted solution as samples, extract the virus DNA of each samples by using Biospin Virus DNA/RNA Extraction Kit and leading brand extraction kit from Q company and T company separately, then detect the DNA concentration by Real-Time PCR. The results are as follows:


            Manual download
          • Biospin Tissue Cell DNA / RNA Extraction Kit MORE
            Product Details:

            This kit can extract DNA and total RNA from cultured animal cells or tissues simultaneously. It adopts special lysis buffer components to release nucleic acid quickly, and uses high-efficient bonding purification system to isolate well integrity and high purity DNA as well as total RNA.The nucleic acid can be applied extensively to PCR, Real-time PCR and the other downstream experiments

            Procedure:

            Product Features:

            1.Convenient:simultaneously extract DNA and total RNA from same sample
            2.Safe: on need of phenol/chloroform extraction
            3.Reliable: suitable for all kinds of downstream molecular biology applications such as PCR and RT-PCR

            Experimental data:

            1、 Qualitative RT-PCR

            RT-PCR result of humo β-actin; Marker: DL2,000

            2、 Quantitative PCR

            Real-time PCR result of humo β-actin


            Manual download
          • Biospin Plant Total RNA Extraction Kit MORE
            Product Details:

            his product is a quick RNA extraction kit without phenol or chloroform. With the unique lysis buffer/ β- mercaptoethanol, the kit can rapidly pyrolysis cells and inactivate RNA enzymes. And the plant RNA reagent helps to bind the polyphenols and polysaccharides in order to remove them by centrifugation. And then by adding ethanol, the RNA will be adsorbed on a silicon membrane inside the spin column at the high salt state. Through a series of quick rinse centrifugal steps, the kit will remove the cellular metabolites, proteins and other impurities entirely. Finally, it will elute the purified RNA from silicon substrate membrane elution in the low salt solution. The high quality kit of the DNA residue-free by adding DNase I with the specific steps of digestion gets no DNA residue but the high-quality RNA


            Product Features:

            1.Specially for purification the total RNA from plants and fungi particularly for the polysaccharides & polyphenol plants
            2.Fast, simple operation by getting the high-quality total RNA within 30 minutes
            3.Without phenol or chloroform extraction
            4.Without cesium chloride gradient centrifugation
            5.Without lithium chloride or ethanol precipitation
            6.Ready for use RNA for a wide range of downstream applications

            Experimental data:

            Manual download
          • Biospin FFPE Tissue Genomic DNA Extraction Kit MORE
            Product Details:

            The kit is for extracting high-quality DNA from paraffin-embedded tissue or formalin-fixed tissue. Using nontoxic dewaxing solution and special lysis buffer formula, it will quickly release and extract sample DNA in good integrity and high purity by high-efficient bondin purification system. After lysising and other special treatment process in order to removing the inhibitor group cross-linked to DNA, the pure DNA can be used for PCR/Real-time PCR、 SPN、 STR and other following applications

            Sample

            Product Features:

            1.Safe and nontoxic dewaxing solution
            2.High integrited
            3.No phemol / chloloform
            4.Stable yield

            Experimental data:

            1. Qualitative PCR

            PCR result of humo β-actin, marker: DL2,000

            2. Quantitative PCR

            Real-time PCR result of humo β-actin


            Manual download
          • Biospin Tissue Genomic DNA Extraction Kit MORE
            Product Details:

            Biospin Tissue Genomic DNA Extraction Kit provides a very simple, fast and economic way to isolate pure high molecular-weight
            genomic DNA from animal tissues. Adopting the Genomic DNA Buffer Set, the simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA in 40min. It doesn't require any expensive equipment, or toxic reagents. In general, 10-30μg genomic DNA can be acquired from up to 50 mg tissue. The pure DNA can be applied extensively in PCR/Real time PCR, sequencing, Southern blot, mutant analysis, SNP and the others


            Item Description
            Sample Volume ≤50mg tissue or cells
            DNA purity OD
            260/OD280: 1.7~2.0
            DNA Length 30~50kb



            Product Features:

            1.Fast and simple; all the work can be finished within 40min
            2.Safe, non-toxin, no need of phenol/chloroform extraction
            3.Sample treating capacity up to 50mg/test
            4.Good DNA integrity

            Kinds of tissues and cells



            Experimental data:

            1. Sample: 20mg mouse brain, 1~10# are same test ,
            Marker: λ DNA /Hind


            Manual download
          • Biospin Gel Extraction Kit MORE
            Product Details:

            Biospin Gel Extraction Kit provides a simple, rapid and effective method to purify DNA fragments from agarose gel in TAE or TBE buffer. DNA fragments ranged from 60bp to 23kb are purified from (up to 3% standard or high/low-melt) gel using Spin column. The recovery rate is 10~55%(<100bp DNA fragments),90~99%(0.1~10kb DNA fragments),20~70%(10kb DNA fragments). Purified DNA can be used directly for kinds of downstream molecular biological experiments such as cloning, sequencing, restriction enzyme digestion and so on

            Item Description
            Sample Volume ≤400mg gel slice
            DNA Fragment Range 60bp~23kb
            Buffer Kind TAE; TBE
            Incubate Temperature 50
            Gel Consistence ≤3



            Product Features:

            1.Fast and simple; all the work can be finished within 15min
            2.Safe, non-toxin, no need of phenol/chloroform extraction
            3.High yield, up to 95
            4.Wide recovery range, DNA fragments ranged from 60bp to 23kb can be recovery.

            Experimental data:

            Gel slice containing DNA fragment
            1.Sample:1007bp PCR product.1~10# are products after extraction. Marker:DL2, 000 .
            Contrast of the absorbance result before and after extraction:
            according to the contrast of gray scale, the yield is 95%.

            Important Notes !

            Please note that the kinds of gel and gel melting point.
            Minimize the size of the gel slice by removing extra agarose,
            that can cause increasing the recovery rate. Use fresh electrophoresis buffer on electrophoresis to improve the recovery rate.And please using high-quality agarose to ensure the recovery rate unaffected by agarose quality.



            Manual download
          • Simply P Virus RNA Extraction Kit MORE
            Product Details:

            Main constituents

             

            Cat#

            BSC56S1

            BSC56M1

            BSC56L1

             

            Ingredients

            Kit Content

            50T

            100T

            200T

            Lysis Buffer

            25 ml

             50 ml

            100 ml

            Salt and Tris-HCl Buffer

            Wash Buffer I

            18ml

            36ml

            72ml

            High-salt solution

            Wash Buffer II

            12ml

            24ml

            48ml

            Low-salt solution

            RElution Buffer

            10ml

             20ml

            40ml

            RNase-free H2O

            Spin Columns

            50

             100

            200

            Plastic parts and nucleic acid adsorption film

            Handbook

            1

             1

            1

             

            PSBuy BSC56S add 12ml Absolute ethanol to 18ml Wash Buffer I before use. add 48ml Absolute ethanol to 12ml Wash buffer II before use.

            Buy BSC56M1 add 24ml Absolute ethanol to 36ml Wash Buffer I before use. add 96ml Absolute ethanol to 24ml Wash buffer II before use.

            Buy BSC56L1 add 48ml Absolute ethanol to 72ml Wash Buffer I before use. add 192ml Absolute ethanol to 48ml Wash buffer II before use.

            Product Features:

            Reagent prepared by the user

            Absolute ethanol (AR).

             Storage and transportation

            1)  The kit can be transported at room temperature.

            2)  The kit has demonstrated stability of 12 months when stored at room temperature.

            Notice

            1)  Lysis Buffer may be precipitated at low temperature, please heated at 56 ℃ for a few minutes to restore the clarification.

            2)  Wash Buffer I and Wash Buffer II add the absolute ethanol as the volume marked on bottle label and mix well.

            Experimental data:

            Purification coronavirus RNA from cat ascites by this kit, real-time PCR result.

            Manual download
          • Simply P Total RNA Extraction kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC52S1

            BSC52M1

            Components

            50Tests

            100Tests

            Solution R1

            10ml

            20ml

            Solution R2

            30ml

            60ml

            Wash Buffer

            25ml

            (add 75ml ethanol before use)

            25ml×2

            (add 75 ml ethanol before use)

            Elution Buffer

            10ml

            20ml

            Spin columns

            50

            100

            Handbook

            1copy

            1copy

            Introduction

                The kit is a ready-to-use reagent for the isolation of total RNA from various sources including whole blood, animal/plant tissue, cultured cell and bacteria. Add Solution R2 to the processed sample and transfer the mixture to spin column, and then total RNA can be easily isolated through several washing and eluting steps.The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-throughput. The pure RNA can be applied extensively in Northern blot, blotting hybridization, poly(A)+ selection、in vitro translation、RNase protect assay, RT-PCR/Real time RT-PCR analysis ,construction cDNA library etc.

            Product Features:

            Storage and transportation

            •  The kit has demonstrated stability of 24 months when stored at room temperature.
            •  The kit can be transported at room temperature.

            Technical Information

            Method

            Time

            Max volume

            Yield

            Spin column

            7~15min

            750µl

            ≥90%

            Apparatus and materials to be prepared by the user

            *  Sterile 1.5ml microcentrifuge tubes                      * 10µl/100µl/1000µl tips

            *  Microcentrifuge capable of 14,000rpm                          * Absolute ethanol

            *  Vortex mixer

            Important note

            Add the ethanol (as the volume marked on bottle label) to the wash buffer and mix them well.

            Experimental data:

            Analysis RNA

            Absorbance analysis of yield and purity

            lPrepare RNA, dilute by 25mM Tris-HCl(pH7.5), RNase-free water or TE buffer in a right factor;

            lZero the spectrophotometer at 260 and 280nm with 25mm Tris-HCl, RNase-free water or TE buffer;

            lMeasure the OD using 100μl the RNA diluent solution, calculate the concentration of RNA as follows:

            Final concentration = (Spec reading A260) × (Dilution factor)  × (Conversion factor A260)

            ð The conversion factor for RNA is 0.040μg/μl per OD260 unit

            ð Notice: the range of Spec reading A2600.1≤OD260≤1.0

            Calculate the purity of RNA as follows: Ratio= (Spec.readingA260)/ (Spec.readingA280)

            ð Ration of 1.8~2.0 are considered ideal purity.

            The low pH will alter the OD measurements between 260 and 280nm, indicating a low purity.

            Picture 1:Animal

            Manual download
          • Biospin Whole Blood Genomic DNA Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC06S1

            BSC06M1

            Component

            Amount

            Amount

            PK solution

            0.5ml

            1.0 ml

            LysisB Buffer

            10.0ml

            20.0 ml

            WB1 Buffer

            11.825ml

            23.65ml

            Wash Buffer

            11.55ml×2

            23.1ml×2

            Elution Buffer

            10ml

            20ml

            Spin column

            50

            100

            Handbook

            1copy

            1copy

            Introduction

                The kit provide a very simple, fast and effective technique for the isolation of pure high–molecular-weight genomic DNA in whole blood which was treated with anticoagulants such as citrate, heparin,  EDTA. The kit is also suitable for the extraction of genome DNA from leukocytes. Based on the remarkable selectivity on genome DNA of Biospin membrane, the simple purification procedure involves only a few steps and allows isolation of high yields of pure genomic DNA within 30 minutes. No any expensive equipments are required, using of toxic or hazardous reagents, such as phenol or chloroform is completely  avoided.In  general,  2~6μg  genomic  DNA  can  be  acquired  from 200μl blood using the kit. The pure DNA can be applied extensively in PCR/Real-time PCR、sequencing、Southern blot、mutant analysis、SNP, and so on.

            Product Features:

            Storage

            • The PK solution must be stored at 2~8℃, other components in the kit may be deposited at room temperature.
            • All components, when stored properly, can keep stable for 18 months.

            Apparatus and Materials should Be Supplied by the User

            *   1.5ml sterile Micro-centrifuge tubes        * 10µl/100µl/1000µl tips

            *   Micro-centrifuge capable of 14,000×g    * Absolute ethanol (>99%)

            *   Vortex mixer

            Experimental data:

            Analysis DNA

            •  Absorbance analysis

            Take some DNA,dilute it into an appropriate concentration with elution buffer.

            Measure it at   OD260   ,OD280  and OD320.

            Equation:concentration(μg/ml)=50×OD260×dilution multiple Target:2.0≥OD260-320/ OD280-320≥1.7

            Notice: 1.0≥OD260≥0.1, the result of ratio is reliable.

            •  Agarose Gel Analysis

            0.8~1% Agarose gel

            Example 1:Blood

            Manual download
          • Biospin Yeast Plasmid DNA Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC01S1G

            BSC01M1G

            Components

            50Tests

            100Tests

            RS Buffer

            30.0ml

            60.0ml

            Resuspension Buffer

            12.5ml

            25ml

            Lysis Buffer

            12.5ml

            25ml

            Neutralization Buffer

            17.5ml

            35ml

            PW Buffer

            25ml

            50ml

            Wash Buffer

            13.0ml

            (add 52ml ethanol before use)

            26.0ml

            (add 104ml ethanol before use)

            Snailase

            260mg×2 (2-8℃)

            260mg×4(2-8℃)

            RNase A

            50ul  (2-8℃)

            100ul (2-8℃)

            Elution Buffer

            10.0ml

            20.0ml

            Spin columns

            50

            100

            Handbook

            1copy

            1copy

            Introduction

                The Kit provides a fast, simple, and cost-effective yeast plasmid miniprep method for routine  molecular biology laboratory applications. Yeast plasmid DNA can be purified from 3–5ml of overnight cultures of yeast. The DNA isolated by this Kit is ready for downstream applications such as transformation, sequencing, PCR/Real-time PCR and other downstream experiments.

            Product Features:

            Storage and Transportation

            • The kit should be stored at room temperature(15~25℃), but the Snailase and the RNase A should be stored at 2~8℃,the Snailase solution should be stored at -20℃.The kit can be stored for up to 12 months by this method. After addition of RNase A, Resuspension Buffer should be stored 2~8℃.
            • The kit can be transported at room temperature.

            Materials to Be Supplied by the User

            * Sterile 1.5ml micro centrifuge tubes     * Absolute ethanol     *β-mercaptoethanol

            Important notes

            1. Please use 1.3 ml RS Buffer to oscillation dissolve each tube snailase, then hold - 20 ℃.
            2. The β-mercaptoethanol should be added into the RS Buffer (10ul/ml) before use. This mixture is  good at room temperature for 1 week.
            3. The RNase A should be all added into the Resuspension Buffer before use, mix and store at 2-8℃.
            4. Add ethanol (as the volume is marked on bottle label) to Wash Buffer then mix well.
            5. If the Lysis Buffer and Neutralization Buffer precipitated, it should be redissolved by warming to 37°C. Please not vortex Lysis Buffer acutely.
            6. Please close the lid immediately after using Lysis Buffer so as to avoid acidification.
            7. The Kit can extract high-quality yeast plasmid DNA from 3-5ml yeast culture overnight cultured.
            Experimental data:

            DNA Analysis

            • Absorbance analysis

            Get some plasmid DNA,diluted in a advisable factor with elution buffer. Survey the OD260, OD280 and OD320

            Expressions:concentration(μg/ml)=50×OD260×dilution fact

            Target: 2.0≥OD260-320/ OD280-320≥1.8

            Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

            • Agarose Gel Analysis
              0.8~1% Agarose gel

            Example 1:Plasmid DNA electrophoresis

            DL15, 000                                    DL2, 000

            Example 2:Enzymatic reactions analysis

                 DL2, 000                                                                                                         DL15, 000

            Manual download
          • Biospin Whole Blood Genomic DNA Midi Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC06S1B

            Components

            10Tests

            PK solution

            1.5ml

            Balance Buffer

            10ml

            5×RCL Buffer

            60ml

            NS Buffer

            20ml

            LysisB Buffer

            20ml

            WB1 Buffer

            17.2ml

            (add22.8ml ethanol before use)

            Wash Buffer

            21ml

            (add 49ml ethanol before use)

            Elution Buffer

            20.0ml

            MidiSpin column

            10

            Handbook

            1 copy

            Introduction

                The kit provides a very simple, fast and effective technique for the isolation of pure high– molecular-weight genomic DNA in 10ml whole blood which was treated with anticoagulants such as citrate, heparin, EDTA. Based on the remarkable selectivity on genome DNA of Midispin membrane, the simple purification procedure involves only a few steps and allows isolation of high yields of pure genomic   DNA within 2hours. No any expensive equipment are required, using of toxic or hazardous reagents, such as phenol or chloroform is completely avoided. In general, 150~250μg genomic DNA can be acquired from 10ml blood using the Kit. The pure DNA can be applied extensively in PCR/Real-time PCR、sequencing、 Southern blot、mutant analysis、SNP, and so on.

            Product Features:

            Storage

            • The PK solution and 5×RCL Buffer must be stored at 2~8℃, other components in the kit may be deposited at room temperature.
            •  All components, when stored properly, can keep stable for 18months.

            Apparatus and Materials should Be Supplied by the User

            *  50ml sterile Micro-centrifuge tubes                 * 10µl/100µl/1000µl tips

            *  Micro-centrifuge capable of 5,000×g               * Absolute ethanol (>99%)

            *  Vortex mixer                                                 * warm bath

            Before Starting:

            (1)     According to the label on the bottle, add ethanol to WB1 buffer and Wash buffer.

            (2)       Add 4 volumes of deionized water to 1 volume of 5×RCL Buffer.

            (3)      Add 1ml Balance Buffer to each MidiSpin column, centrifuge at 5,000 x g for 3 min. Discard flow-through.

            Experimental data:

            Analysis DNA

            • Absorbance analysis

            Take some DNA,dilute it into an appropriate concentration with elution buffer. Measure it at OD260,OD280 and OD320.

            Equation:concentration(μg/ml)=50×OD260×dilution multiple

            target:2.0≥OD260-320/ OD280-320≥1.7

            Notice: 1.0≥OD260≥0.1, the result of ratio is reliable.

            •  Agarose Gel Analysis

            0.8~1% Agarose gel

            Manual download
          • Biospin Whole Blood Genomic DNA Maxi Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC06S1C

            Components

            10Tests

            PK solution

            5ml

            Balance Buffer

            20ml

            LysisB Buffer

            70ml×2

            WB1 Buffer

            25.8ml

            (add 34.2ml ethanol before use)

            Wash Buffer

            64ml

            (add 96ml ethanol before use)

            Elution Buffer

            20.0ml

            MaxiSpin column

            10 tubes

            Handbook

            1 copy

            Introduction

                The kit provides a very simple, fast and effective technique for the isolation of pure high– molecular-weight genomic DNA in 10ml whole blood based on the remarkable selectivity on genome DNA of Maxispin membrane, the simple purification procedure involves only a few steps and allows isolation of high yields of pure genomic DNA within 2hours. No any expensive equipment are required, using of  toxic or  hazardous  reagents,  such  as  phenol  or  chloroform  is  completely  avoided.  In  general, 150~250μg genomic DNA can be acquired from 10ml blood using the Kit. The pure DNA can be applied extensively in PCR/Real-time PCR、sequencing、Southern blot、mutant analysis、SNP, and so on.

            Product Features:

            Storage

            • The PK solution must be stored at 2~8℃, other components in the kit may be deposited at room temperature.
            • All components, when stored properly, can keep stable for 18months.

            Apparatus and Materials should Be Supplied by the User

            *  50ml sterile Micro-centrifuge tubes       * 10µl/100µl/1000µl tips

            *  Micro-centrifuge capable of 5,000×g     * Absolute ethanol (>99%)

             *  Vortex mixer                                      * warm bath

            Before Starting:

            1. According to the label on the bottle, add ethanol to WB1 buffer and Wash buffer.
            2. Add 2 ml Balance Buffer to each MaxiSpin column, centrifuge at 5,000 x g for 3 min. Discard flow-through.

            Note:Each elution typically yields 60%of DNA bound to the column. To obtain DNA at higher concentrations, please suck the liquid collected in the collection tube again to the column. Incubate at room temperature for 2 minutes. Centrifuge at 4,000×g for 3 minutes. The DNA in the collection tube is ready for further analysis. If the isolated DNA sample is not going to be tested on the same day, freeze it at -20℃.

            Experimental data:

            Analysis DNA

            •  Absorbance analysis

            Take some DNA,dilute it into an appropriate concentration with elution buffer. Measure it at OD260,OD280 and OD320.

            Equation:concentration(μg/ml)=50×OD260×dilution multiple

            target:2.0≥OD260-320/ OD280-320≥1.7

            Notice: 1.0≥OD260≥0.1, the result of ratio is reliable.

            • Agarose Gel Analysis 

            0.8~1% Agarose gel

            Example 12ml whole blood


            Example 25ml Blood clots


            Manual download
          • Biospin Virus RNA Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC62S1

            BSC62M1

            Components

            50Tests

            100Tests

            RLysis Buffer

            15ml

            30ml

            RD Buffer

            17.5ml

            35ml

            DNase Stop Buffer

            5.0ml

            (add 6.5ml ethanol before use)

            10.0ml

            (add 13ml ethanol before use)

            Wash Buffer

            20ml

            (add 30ml ethanol before use)

            40ml

            (add 60ml ethanol before use)

            RElution Buffer

            10ml

            20ml

            Spin Columns

            50

            100

            Handbook

            1copy

             1copy

            Introduction

                The kit is a ready-to-use reagent for the isolation of Virus RNA from different type’s sample. Add RLysis Buffer to the processed sample and adding alcohol will bind RNA to spin column. Then RNA can be easily isolated through several washing and eluting steps.

                The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-throughput. The pure RNA can be applied extensively in Northern blot, blotting hybridization, in vitro translation, RNase protect assay, RT-PCR/Real-time RT-PCR analysis, construction cDNA library etc.

            Product Features:

            Storage and transportation

            • The kit has demonstrated stability of 24 months when stored at room temperature.
            • The kit can be transported at room temperature.

            Technical Information

            Sample

            Amount

            Animal tissue

            ≤30mg

            Culture cells

            ≤1×108

            White blood cells

            ≤from 5ml whole blood

            For liquid sample

            ≤100μl

            Apparatus and materials to be prepared by the user

            *  Sterile 1.5ml microcentrifuge tubes            * 10µl/100µl/1000µl tips

            *  Microcentrifuge capable of 14,000rpm       * 70℃ Water bath      * Vortex mixer

            *  Liquid nitrogen (or ice bath)     * Optional: β-Me        * PBS          *Trypsination

            *  Red Blood Cells Lysis Buffer(Cat#BSA06M1)         * Absolute alcohol

            Important note

            • RD Buffer may be precipitated at low temperature, the heated at 37 ℃ for a few minutes, to restore the clarification.
            • DNase Stop Buffer Add the alcohol as the volume marked on bottle label and mix well.
            • Wash Buffer Add the alcohol as the volume marked on bottle label and mix well.
            Experimental data:

            Analysis RNA

            ExampleHCV Real-time RT-PCR  (Sample HCV concentration:copies/ml)

            Manual download
          • Biospin Total RNA Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC63S1

            BSC63M1

            Components

            50Tests

            100Tests

            RLysis Buffer

            8.75ml

            17.5ml

            RD Buffer

            17.5ml

            35ml

            DNase Buffer

            2ml

            4ml

            MnCl2

            450ul

            900ul

            DNase I

            50ul(stored at -20℃)

            100ul(stored at -20℃)

            DNase Stop Buffer

            5 ml

            (add 6.5 ml ethanol before use)

            10 ml

            (add 13 ml ethanol before use)

            Wash Buffer

            32 ml

            (add 48ml ethanol before use)

            32 ml

            (add 48ml ethanol before use)×2

            RElution Buffer

            10ml

            20ml

            Spin columns

            50

            100

            Handbook

            1 copy

            1 copy

            Introduction

                The kit is a ready-to-use reagent for the isolation of total RNA from animal tissues, cells, bacteria and others (plant tissues are not recommended). Add RLysis Buffer to the processed sample.RD buffer will remove the protein, then adding alcohol will bind RNA to spin column. The DNA will be destroyed by the DNase I reaction. Then RNA can be easily isolated through several washing and eluting steps.

                The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-throughput. The pure RNA can be applied extensively in Northern blot, blotting hybridization, in vitro translation,RNase protect assay, RT-PCR/Real time RT-PCR analysis, construction cDNA library etc.

            Product Features:

            Storage and transportation

            • The kit has demonstrated stability of 18 months when the DNase I should be stored at -20 ℃, others at room temperature.
            • The kit can be transported at room temperature.

            Apparatus and materials to be prepared by the user

            *  Sterile 1.5ml centrifuge tubes                       * 10µl/100µl/1000µl tips

            *  Microcentrifuge capable of 14,000rpm           * Absolute alcohol

            *  Vortex mixer                                              * β- mercaptoethanol

             Important note

            • DNase Stop Buffer: Add the alcohol as the volume marked on bottle label and mix well.
            • Wash Buffer: Add the alcohol as the volume marked on bottle label and mix well.
            Experimental data:

            Analysis RNA

            • Absorbance analysis

            Get some RNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.

            Expressions:concentration(μg/ml)=40×OD260×dilution fact

            Target:2.0≥OD260-320/ OD280-320≥1.7

            Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

            • Agarose Gel Analysis

            Manual download
          • Biospin SwabGen DNA Extraction Kit MORE
            Product Details:

             Kit Components

             

            Cat#

            BSC28S1

            BSC28M1

            Components

            50Tests

            100Tests

            PK solution

            500ul

            1000ml

            LysisB Buffer

            30ml

            60ml

            WB1 Buffer

            12ml

            (add 17ml ethanol before use)

            24ml

            (add 34ml ethanol before use)

            Wash Buffer

            18ml

            (add 42ml ethanol before use)

            36ml

            (add 84ml ethanol before use)

            Elution Buffer

            10 ml

            20 ml

            Spin column

            50 tubes

            100 tubes

            Handbook

            1copy

            1copy

            Introduction

                The kit provides a very simple, fast and effective technique for the isolation of pure high– molecular-weight genomic DNA in Oral swabs. Based on the remarkable selectivity on genome DNA of Biospin membrane, the simple purification procedure involves only a few steps and allows isolation of high yields of pure genomic DNA within 30 minutes. No any expensive equipments are required, using of  toxic or  hazardous  reagents,  such  as  phenol  or  chloroform  is  completely  avoided.  In  general,   0.5~3.5μg genomic DNA can be acquired using the Kit. The pure DNA can be applied extensively in PCR/Real-time PCR、sequencing、Southern blot、mutant analysis、SNP, and so on.

            Product Features:

            Storage and transportation

            • The PK solution must be stored at 2~8℃, other components in the kit may be deposited at room temperature.
            • All components, when stored properly, can keep stable for 18months.
            • The kit can be transported at room temperature.

            Apparatus and materials to be prepared by the user

            *  1.5ml and 2.0ml sterile micro-centrifuge tubes   * 10µl/100µl/1000µl tips

            *  Micro-centrifuge capable of 12,000×g                * Absolute ethanol (>99%)

            *  Vortex mixer                                                  * Warm bath or metal bath

            Important note

            Add the ethanol (as the volume marked on bottle label) to WB1 buffer and wash buffer and mix them well.

            Experimental data:

            Analysis DNA

            •  Absorbance analysis

             Take some DNA,dilute it into an appropriate concentration with elution buffer.

             Measure it at OD260,OD280 and OD320.

            Equation:concentration(μg/ml)=50×OD260×dilution multiple

            target:2.0≥OD260-320/ OD280-320≥1.7

            Notice: 1.0≥OD260≥0.1, the result of ratio is reliable.

            • Agarose Gel Analysis 0.8~1% Agarose gel

             SYBR Green Real-time PCR

            Manual download
          • Biospin Polyacrylamide Gel DNA Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC15S1

            BSC15M1

            Components

            50Tests

            100Tests

            PE Buffer

            10 ml

            20 ml

            PEB Buffer

            20 ml

            40 ml

            PWash Buffer

            18.9ml

            37.8ml

            Elution Buffer

            10ml

            20ml

            Spin Column

            50

            100

            Handbook

            1copy

            1copy

            Introduction

                The kit provides a simple, rapid and effective method for purification of DNA fragments from polyacrylamide gel. The simple purification procedure is based on the remarkable selectivity of Biospin membrane. There need few steps to finish extraction without expensive equipment, as completely avoids using toxic and hazardous reagents such as phenol and chloroform. Purified DNA can be used directly for kinds of downstream molecular biological experiments such as cloning, sequencing, PCR, restriction enzyme digestion, and so on.

            Product Features:

            Storage and transportation

            • The kit should be stored dry at room temperature(15~25℃), The kit can be stored for up to 18 months if all components are kept properly.
            • The kit can be transported at room temperature.

            Technical Information

             Method

            Column volume

            DNA size range

            Electrophoresis buffer

            Incubate temperature

            Elution recovery

            Spin column

                 750µl

            100bp~10kb

            TAE/TBE buffer

            65℃

            ≥99%

            Apparatus and Materials to Be Supplied by the User

            * Sterile 1.5 or 2.0 ml microcentrifuge tubes        * 10µl/100µl/1000µl tips

            *  Absolute ethanol    * Microcentrifuge capable of 14,000g * Vortex mixer   * Water bath or cooling & heating block

            Preparation

             

            1. This product cannot be used with PAGE with urea.
            2. Add ethanol as per the volume marked on label of bottle into Wash Buffer and then mix well.
            3. If the gel slice is very big, please increase the dosage of PE Buffer properly but on more than 200μl, thus to guarantee that the gel slice is immerged completely.
            4. The suitable volume of elution buffer for the kit is 50µl. The dosage of elution buffer can be adjusted according to practical experimental situation.
            5. Please wash the fixed gel by water or 10mM Tris-HCl (pH7.0) for 2 or 3 times if the gel is fixed by acidic reagent such as acetic acid.
          • Biospin Plasmid DNA Maxi Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC01S1C

            BSC01M1C

            Components

            10Tests

            25Tests

            Balance Buffer

            20ml

            50ml

            Resuspension Buffer

            100ml

            250ml

            Lysis Buffer

            100ml

            250ml

            Neutralization Buffer

            120ml

            100ml

            Washing Buffer

            50mlх2

            (add 75ml ethanol before use)

            104mlх2

            (add 156ml ethanol before use)

            Elution Buffer

             60ml

            150ml

            RNase A

            1 tube

            1 tube

            Max Spin column

            10 tubes

            25 tubes

            Handbook

            1 copy

            1 copy

            Introduction

                 This kit allows the high efficient binding of DNA to our spin matrix while proteins and  other  impurities are removed by wash buffer. Nucleic acids are easily eluted with sterile water or Elution Buffer. This kit is designed for fast and efficient purification of plasmid DNA from 150 to 200 ml of E. coli culture. The maxi column has a plasmid DNA binding capacity of 1 mg.

                The purified plasmid DNA is ready for downstream applications such as transfection of robust cells such as HEK293, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations.

            Product Features:

            Storage and transportation

            •  The kit has demonstrated stability of 18 months when the RNase A should be stored at 2-8℃,others at room temperature.
            • The kit can be transported at room temperature.
            •  Resuspension Buffer should be stored at 2°C-8℃ once RNase A is added.

            Apparatus and materials to be prepared by the user

             *  50ml centrifuge tubes                            * 10µl/100µl/1000µl tips

             *  Centrifuge capable of 14,000g               * Absolute alcohol

            Important note

            1)Spin down RNase A vial briefly. Add the RNase A solution to Resuspension Buffer and mix well before use. Resuspension Buffer should be stored at 2°C-8℃ once RNase A is added.

            2)Wash Buffer: Add the alcohol as the volume marked on bottle label and mix well.

            3)Lysis Buffer precipitates below room temperature. It is critical to warm up the buffer at 50°C to dissolve the precipitates before use.

            4)Neutralization Buffer may form precipitates below 10°C, warm up at 37°C to dissolve the precipitates before use.

            5)Carry out all centrifugations at room temperature.

            6) Add 2ml Balance Buffer to each MaxiSpin column, incubate at room temperature for 2 mins,centrifuge at 5,000 x g for 3 min. Discard flow-through. It can improve the yield of DNA significantly.

          12
          Privacy clause

          Respect the user's privacy is a basic policy of Hangzhou Bioer user service. Hangzhou Boier will not disclosure user information to third parties or disclose their registration and preservation of non disclosure in Hangzhou Bioer platform, except in the following situations: (1)User's improper behavior caused leakage of privacy information ; (2)Caused by network, hacker attack, computer virus, government regulation, the reasons of the leakage, lost, stolen or tampered with other relevant legal requirements; (3)Law-enforcement agency asked Hangzhou Bioer to provide privacy information of the user; (4)Emergency situation when public life and property security is under threate.

          Exemption clause

          Hangzhou Bioer technology does not guarantee the accuracy, integrity and reliability of any content on this website and any content that may use these results. Hangzhou Bioer technology and related people can not guarantee that you can enter, browse and use this website at any time; Hangzhou Bioer technology on this website and its contents can not use and do not take any responsibility. The site links to other websites that Hangzhou itself is not recognized or undertake other Bioer technology content or the use of responsibility. Hangzhou Bioer technology and related people to enter, browse and use of the site, from the website to download any content or by any link to this site to other sites caused by viruses or other destructive programs on your computer system and any other software and hardware, IT system or property damage or loss does not bear any responsibility. Hangzhou Bioer technology and the relevant people do not bear any responsibility caused by the third party using illegal means to obtain the password, data and content into the site of any direct and indirect damage or loss . The final interpretation belong to Hangzhou Bioer Technology Co. Ltd.

          Hangzhou Bioer reserves the right to correct, modify and update this statement at any time.

          Copyright

          This website from Hangzhou Bioer Technology Co. Ltd. (hereinafter referred to as "Hangzhou Bioer") the creation of any content of this website (including but not limited to text, data, graphics, images, voice or video) are copyright or related rights belong to Hangzhou Bioer. Hangzhou Bioer or related rights without prior written permission, you shall not in any way without permission to copy, reconstruction, spread, publishing, reprint, repost or display the contents of this website. Any unauthorized use of this site may violate the "Copyright Law of People's Republic of China " and other relevant laws and regulations and international conventions, Hangzhou Bioer fully pursue appropriate legal responsibility right.

          Contact Us

          Address: 1192 Bin An Rd, Hi-tech (Binjiang)District,Hangzhou,310053,P. R. China

          Tel:0571-87774567

          99热精品国产三级在线,成熟女人色惰片免费视频,国产精品乱码高清在线观看,韩国三级香港三级日本三级l