Spin Column Method

• Biospin Total RNA Extraction Kit II MORE
  Product Details：

  This kit is a column-type total RNA extraction kit based on the improved lysis and binding reagent components, which improves the lysis and binding performance of the reagent. The addition of chloroform realizes the separation of impurities such as RNA from DNA and protein, and optimizes the column membrane to improve its ability to adsorb RNA. After multiple washings, impurities are effectively removed to the greatest extent, and high-purity and high-quality total RNA is obtained. Nucleic acid can be extracted from a wide range of samples such as blood, animal and plant tissues, various microorganisms and cultured cells. High-quality nucleic acid products can be directly used in downstream molecular experiments such as Northern Blot, RT-qPCR, and NGS.

  

  Product Features：

  1.  High purity

  2.  No DNA residue

  3.  Sufficient protein removal

  4.  Simple operation

  Experimental data:

  Case 1 

  1. Extract 1mL (10⁶/mL) of monolayer cultured cells, 100μL of Elution buffer, use Nanodrop 2000 to measure the concentration and 1% agarose gel electrophoresis to detect the integrity of nucleic acid bands, and compare with similar brands reagents.

  Reagent brand	Concentration (ng/μL)	260/280	260/230
  Bioer company	361.4	2.06	1.82
  T company	352	2.08	1.9
  N company	356.3	2.06	1.72

  

  Case 2

  Extract 50mg of the leaf tissues of  Buxus microphylla and Ophiopogon japonicus, 100μL Elution Buffer, use Nanodrop 2000 to measure the concentration and 1% agarose gel electrophoresis to detect the integrity of nucleic acid bands, and compare with similar brands reagents.

  Sample	T company			Bioer company
  	Concentration (ng/μL)	260/280	260/230	Concentration (ng/μL)	260/280	260/230
  Buxus microphylla	204.3	2	1.03	228.9	2.12	1.6
  Ophiopogon japonicus	251.8	2.13	2.15	301.5	2.12	1.26

   

  

  The results show that the extraction efficiency of Bioer's kit is better than that of similar products of other brands.

   

  Manual download
• Biospin Virus DNA Extraction Kit II MORE
  Product Details：

  This kit uses a unique lysis solution to lyse the virus, and is suitable for extracting viral DNA from ≤200μL plasma, serum, cell-free body fluid, and cultured animal cell supernatants. The purified viral DNA can be widely used in Real-time PCR, Sequencing, Southern Blot, Mutant Analysis, SNP and other common downstream experiments in molecular biology.

   

  

  Product Features：

  1. Fast and efficient: the purification time of virus DNA is less than 30 minutes;
  2. Easy to operate: no need for Carrier RNA;
  3. Safe and non-toxic: safe and environmentally friendly, without irritating and toxic ingredients;
  4. High purity: impurities are removed cleanly and can be directly applied to downstream analysis and detection

  Experimental data:

  Case 1

  Extract DNA (5~10⁵IU/mL) from serially diluted serum samples of hepatitis B virus (HBV) positive serum, and perform high-sensitivity HBV fluorescence quantitative PCR detection. The results are as follows:

   

  Samples（IU/ml）	CT Value
  E5	22.69
  E4	25.34
  E3	28.71
  E2	31.3
  25	33.38
  10	33.88
  5	36.09
  Negative control	-

  

   

  Case 2： 

  A customer used the BIOER Biospin Virus DNA Extraction Kit II to compare with similar products from S company, extract 4 environmental samples, and perform fluorescence quantitative PCR amplification at the same time to detect the presence of African swine fever virus in the samples. The results are as follows:

  Ct value Sample	Bioer Ct value	S Company Ct value
  1	27.61	29.07
  2	28.26	30.94
  3	No Ct	No Ct
  4	No Ct	No Ct

  The above results show that the BIOER Biospin virus DNA extraction kit II product is superior to the similar products of S company.

  Manual download
• SimplyP Animal pathogens DNA/RNA Extraction Kit MORE
  Product Details：

  This product provides a set of methods for quickly extracting DNA and RNA viruses from tissue homogenate supernatant and serum, plasma, whole blood and ascites samples. The extracted nucleic acid can be directly used for PCR, RT-PCR/Real-time PCR Wait for downstream experiments.

   

  

  Product Features：

  1、The sample can be extracted within 11 minutes.

  2、Less steps: 4 steps can be completed.

  3、New technology: high sensitivity can be achieved without using reagents such as proteinase K and Carrier RNA.

  4、Wide range of applications: suitable for extracting various DNA and RNA viruses

  Experimental data:

  Case 1： 

  Extract cat feces samples for real-time PCR amplification of cat coronavirus:

  Result Sample	Ct Value
  1	17.79
  2	21.4
  3	25.18
  4	25.28
  5	29.29
  6	33.66
  7	NoCt

   

   

   

   

   

  

  1：Original template ； 2：10^-1； 3：Positive control；4：10^-2；5；10^-3；6：10^-4；7：Negative control

   

  Case 2： 

  Extract cat snout and nose swab samples for real-time PCR amplification of feline herpes virus: 

  Result Sample	Ct Value
  Original liquid	20.26	20.08
  10 times dilution	23.42	23.57
  100 times dilution	27.09	26.93
  1000 times dilution	30.45	30.55
  10000 times dilution	34.06	34.17
  Negative control	NoCt	NoCt

   

   

   

   

   

  

  Manual download
• Biospin Virus DNA/RNA Extraction Kit MORE
  Product Details：

  The Biospin Virus DNA/RNA Extraction Kit is designed for the rapid and efficient isolation and purification of high quality virus nucleic acid from a variety of samples. It takes about only 30 minutes for the total procedure.
  The obtained virus nucleic acid can be used directly for a broad range of downstream applications, such as qPCR, NGS, etc
  
  
  
  

  Features	Specifications
  Format	Spin Column
  Technology	Silica membrane technology
  Sample	Serum, Plasma, Whole blood, Swabs, Saliva, Body fluid, Tissue, Feces, BALF, etc.
  Sample Volume	200 μL
  Elution Volume	50-100 μL
  Processing	Manual (Centrifugation)
  Processing Time	30 minutes
  Yield	High purity viral DNA or Viral RNA
  Storage Conditions	2-30°C (Protease K: 2-8°C)
  Shelf life	12 months

  
  

  Product Features：

  1.Rapid and Reliable: Fast procedures and easy to use
  2.High Sensitivity: DNA: 10 IU/mL, RNA: 50 IU/mL
  3.Versatile: Nucleic acid of various virus, e.g. SARS-CoV-2, Hepatitis, etc. in different samples, such as Serum, Plasma, Whole blood, Swabs, Saliva,Body fluid, Tissue, Feces, BALF, etc. for a broad range of down stream applications
  4.Safe: Toxic free
  
  

  Experimental data:

  Case 1 To Extract RNA from Coronavirus

  Gradient dilute the supernatant of fecal sample which contain Coronavirus, using the original and the diluted solution as samples, extract the Coronavirus RNA of each samples by using Biospin Virus DNA/RNA Extraction Kit and a leading brand extraction kit from Q company separately, then detect the Coronavirus RNA concentration by Real-Time RT-PCR. The results are as follows:
  
  

  Case 4 To Extract DNA from African Swine Fever Virus

  Gradient dilute the tissue homogenate that contains African Swine Fever Virus, using the original and the diluted solution as samples, extract the virus DNA of each samples by using Biospin Virus DNA/RNA Extraction Kit and leading brand extraction kit from Q company and T company separately, then detect the DNA concentration by Real-Time PCR. The results are as follows:

  
  

  Manual download
• Biospin Tissue Cell DNA / RNA Extraction Kit MORE
  Product Details：

  This kit can extract DNA and total RNA from cultured animal cells or tissues simultaneously. It adopts special lysis buffer components to release nucleic acid quickly, and uses high-efficient bonding purification system to isolate well integrity and high purity DNA as well as total RNA.The nucleic acid can be applied extensively to PCR, Real-time PCR and the other downstream experiments
  
  Procedure：

  

  Product Features：

  1.Convenient:simultaneously extract DNA and total RNA from same sample
  2.Safe: on need of phenol/chloroform extraction
  3.Reliable: suitable for all kinds of downstream molecular biology applications such as PCR and RT-PCR
  
  

  Experimental data:

  1、 Qualitative RT-PCR
  
  

  RT-PCR result of humo β-actin； Marker: DL2,000
  
  2、 Quantitative PCR

  

  Real-time PCR result of humo β-actin
  
  
  

  Manual download
• Biospin Plant Total RNA Extraction Kit MORE
  Product Details：

  his product is a quick RNA extraction kit without phenol or chloroform. With the unique lysis buffer/ β- mercaptoethanol, the kit can rapidly pyrolysis cells and inactivate RNA enzymes. And the plant RNA reagent helps to bind the polyphenols and polysaccharides in order to remove them by centrifugation. And then by adding ethanol, the RNA will be adsorbed on a silicon membrane inside the spin column at the high salt state. Through a series of quick rinse centrifugal steps, the kit will remove the cellular metabolites, proteins and other impurities entirely. Finally, it will elute the purified RNA from silicon substrate membrane elution in the low salt solution. The high quality kit of the DNA residue-free by adding DNase I with the specific steps of digestion gets no DNA residue but the high-quality RNA
  
  
  

  Product Features：

  1.Specially for purification the total RNA from plants and fungi particularly for the polysaccharides & polyphenol plants
  2.Fast, simple operation by getting the high-quality total RNA within 30 minutes
  3.Without phenol or chloroform extraction
  4.Without cesium chloride gradient centrifugation
  5.Without lithium chloride or ethanol precipitation
  6.Ready for use RNA for a wide range of downstream applications
  

  Experimental data:

  

  Manual download
• Biospin FFPE Tissue Genomic DNA Extraction Kit MORE
  Product Details：

  The kit is for extracting high-quality DNA from paraffin-embedded tissue or formalin-fixed tissue. Using nontoxic dewaxing solution and special lysis buffer formula, it will quickly release and extract sample DNA in good integrity and high purity by high-efficient bondin purification system. After lysising and other special treatment process in order to removing the inhibitor group cross-linked to DNA, the pure DNA can be used for PCR/Real-time PCR、 SPN、 STR and other following applications
  
  Sample
  
  

  Product Features：

  1.Safe and nontoxic dewaxing solution
  2.High integrited
  3.No phemol / chloloform
  4.Stable yield
  
  

  Experimental data:

  1. Qualitative PCR
  
  

  PCR result of humo β-actin, marker: DL2,000
  
  2. Quantitative PCR

  

  Real-time PCR result of humo β-actin
  
  
  

  Manual download
• Biospin Tissue Genomic DNA Extraction Kit MORE
  Product Details：

  Biospin Tissue Genomic DNA Extraction Kit provides a very simple, fast and economic way to isolate pure high molecular-weight
  genomic DNA from animal tissues. Adopting the Genomic DNA Buffer Set, the simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA in 40min. It doesn't require any expensive equipment, or toxic reagents. In general, 10-30μg genomic DNA can be acquired from up to 50 mg tissue. The pure DNA can be applied extensively in PCR/Real time PCR, sequencing, Southern blot, mutant analysis, SNP and the others
  
  

  Item	Description
  Sample Volume	≤50mg tissue or cells
  DNA purity	OD 260/OD280: 1.7~2.0
  DNA Length	30~50kb

  
  
  

  Product Features：

  1.Fast and simple; all the work can be finished within 40min
  2.Safe, non-toxin, no need of phenol/chloroform extraction
  3.Sample treating capacity up to 50mg/test
  4.Good DNA integrity
  
  Kinds of tissues and cells
  
  
  
  

  Experimental data:

  1. Sample: 20mg mouse brain, 1~10# are same test ,
  Marker: λ DNA /Hind Ⅲ
  
  

  Manual download
• Biospin Gel Extraction Kit MORE
  Product Details：

  Biospin Gel Extraction Kit provides a simple, rapid and effective method to purify DNA fragments from agarose gel in TAE or TBE buffer. DNA fragments ranged from 60bp to 23kb are purified from (up to 3% standard or high/low-melt) gel using Spin column. The recovery rate is 10~55%(<100bp DNA fragments),90~99%(0.1~10kb DNA fragments),20~70%(﹥ 10kb DNA fragments). Purified DNA can be used directly for kinds of downstream molecular biological experiments such as cloning, sequencing, restriction enzyme digestion and so on
  
  

  Item	Description
  Sample Volume	≤400mg gel slice
  DNA Fragment Range	60bp~23kb
  Buffer Kind	TAE; TBE
  Incubate Temperature	50℃
  Gel Consistence	≤3

  
  
  

  Product Features：

  1.Fast and simple; all the work can be finished within 15min
  2.Safe, non-toxin, no need of phenol/chloroform extraction
  3.High yield, up to 95％
  4.Wide recovery range, DNA fragments ranged from 60bp to 23kb can be recovery.
  
  

  Experimental data:

  Gel slice containing DNA fragment
  1.Sample:1007bp PCR product.1~10# are products after extraction. Marker:DL2, 000 .
  Contrast of the absorbance result before and after extraction:
  according to the contrast of gray scale, the yield is 95%.
  

  Important Notes ！

  Please note that the kinds of gel and gel melting point.
  Minimize the size of the gel slice by removing extra agarose,
  that can cause increasing the recovery rate. Use fresh electrophoresis buffer on electrophoresis to improve the recovery rate.And please using high-quality agarose to ensure the recovery rate unaffected by agarose quality.
  
  
  

  Manual download
• Simply P Virus RNA Extraction Kit MORE
  Product Details：

  Main constituents

   

  Cat#	BSC56S1	BSC56M1	BSC56L1	Ingredients
  Kit Content	50T	100T	200T
  Lysis Buffer	25 ml	50 ml	100 ml	Salt and Tris-HCl Buffer
  Wash Buffer I	※18ml	※36ml	※72ml	High-salt solution
  Wash Buffer II	※12ml	※24ml	※48ml	Low-salt solution
  RElution Buffer	10ml	20ml	40ml	RNase-free H2O
  Spin Columns	50	100	200	Plastic parts and nucleic acid adsorption film
  Handbook	1	1	1

  PS：Buy BSC56S add 12ml Absolute ethanol to ※18ml Wash Buffer I before use. add 48ml Absolute ethanol to ※12ml Wash buffer II before use.

  Buy BSC56M1 add 24ml Absolute ethanol to ※36ml Wash Buffer I before use. add 96ml Absolute ethanol to ※24ml Wash buffer II before use.

  Buy BSC56L1 add 48ml Absolute ethanol to ※72ml Wash Buffer I before use. add 192ml Absolute ethanol to ※48ml Wash buffer II before use.

  Product Features：

  Reagent prepared by the user

  Absolute ethanol (AR).

   Storage and transportation

  1)  The kit can be transported at room temperature.

  2)  The kit has demonstrated stability of 12 months when stored at room temperature.

  Notice

  1)  Lysis Buffer may be precipitated at low temperature, please heated at 56 ℃ for a few minutes to restore the clarification.

  2)  Wash Buffer I and Wash Buffer II add the absolute ethanol as the volume marked on bottle label and mix well.

  Experimental data:

  Purification coronavirus RNA from cat ascites by this kit, real-time PCR result.

  

  Manual download
• Simply P Total RNA Extraction kit MORE
  Product Details：

  Kit Components

  Cat#	BSC52S1	BSC52M1
  Components	50Tests	100Tests
  Solution R1	10ml	20ml
  Solution R2	30ml	60ml
  Wash Buffer	25ml (add 75ml ethanol before use)	25ml×2 (add 75 ml ethanol before use)
  Elution Buffer	10ml	20ml
  Spin columns	50	100
  Handbook	1copy	1copy

  Introduction

      The kit is a ready-to-use reagent for the isolation of total RNA from various sources including whole blood, animal/plant tissue, cultured cell and bacteria. Add Solution R2 to the processed sample and transfer the mixture to spin column, and then total RNA can be easily isolated through several washing and eluting steps.The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-throughput. The pure RNA can be applied extensively in Northern blot, blotting hybridization, poly(A)+ selection、in vitro translation、RNase protect assay, RT-PCR/Real time RT-PCR analysis ,construction cDNA library etc.

  Product Features：

  Storage and transportation

  •  The kit has demonstrated stability of 24 months when stored at room temperature.
  •  The kit can be transported at room temperature.

  Technical Information

  Method	Time	Max volume	Yield
  Spin column	7~15min	750µl	≥90％

  Apparatus and materials to be prepared by the user

  *  Sterile 1.5ml microcentrifuge tubes                      * 10µl/100µl/1000µl tips

  *  Microcentrifuge capable of 14,000rpm                          * Absolute ethanol

  *  Vortex mixer

  Important note

  Add the ethanol (as the volume marked on bottle label) to the wash buffer and mix them well.

  Experimental data:

  Analysis RNA

  Absorbance analysis of yield and purity

  lPrepare RNA, dilute by 25mM Tris-HCl（pH7.5）, RNase-free water or TE buffer in a right factor；

  lZero the spectrophotometer at 260 and 280nm with 25mm Tris-HCl, RNase-free water or TE buffer；

  lMeasure the OD using 100μl the RNA diluent solution, calculate the concentration of RNA as follows:

  Final concentration = (Spec reading A260) × (Dilution factor)  × (Conversion factor A260)

  ð The conversion factor for RNA is 0.040μg/μl per OD260 unit

  ð Notice: the range of Spec reading A260： 0.1≤OD260≤1.0

  Calculate the purity of RNA as follows: Ratio= (Spec.readingA260)/ (Spec.readingA280)

  ð Ration of 1.8~2.0 are considered ideal purity.

  （The low pH will alter the OD measurements between 260 and 280nm, indicating a low purity.）

  Picture 1：Animal

  

  Manual download
• Biospin Whole Blood Genomic DNA Extraction Kit MORE
  Product Details：

  Kit Components

  Cat#	BSC06S1	BSC06M1
  Component	Amount	Amount
  PK solution	0.5ml	1.0 ml
  LysisB Buffer	10.0ml	20.0 ml
  WB1 Buffer	11.825ml	23.65ml
  Wash Buffer	11.55ml×2	23.1ml×2
  Elution Buffer	10ml	20ml
  Spin column	50	100
  Handbook	1copy	1copy

  Introduction

      The kit provide a very simple, fast and effective technique for the isolation of pure high–molecular-weight genomic DNA in whole blood which was treated with anticoagulants such as citrate, heparin,  EDTA. The kit is also suitable for the extraction of genome DNA from leukocytes. Based on the remarkable selectivity on genome DNA of Biospin membrane, the simple purification procedure involves only a few steps and allows isolation of high yields of pure genomic DNA within 30 minutes. No any expensive equipments are required, using of toxic or hazardous reagents, such as phenol or chloroform is completely  avoided.In  general,  2～6μg  genomic  DNA  can  be  acquired  from 200μl blood using the kit. The pure DNA can be applied extensively in PCR/Real-time PCR、sequencing、Southern blot、mutant analysis、SNP, and so on.

  Product Features：

  Storage

  • The PK solution must be stored at 2～8℃, other components in the kit may be deposited at room temperature.
  • All components, when stored properly, can keep stable for 18 months.

  Apparatus and Materials should Be Supplied by the User

  *   1.5ml sterile Micro-centrifuge tubes        * 10µl/100µl/1000µl tips

  *   Micro-centrifuge capable of 14,000×g    * Absolute ethanol (>99%)

  *   Vortex mixer

  Experimental data:

  Analysis DNA

  •  Absorbance analysis

  Take some DNA，dilute it into an appropriate concentration with elution buffer.

  Measure it at   OD260   ，OD280  and OD320.

  Equation：concentration（μg/ml）＝50×OD260×dilution multiple Target：2.0≥OD260-320/ OD280-320≥1.7

  Notice: 1.0≥OD260≥0.1, the result of ratio is reliable.

  •  Agarose Gel Analysis

  0.8～1％ Agarose gel

  Example 1：Blood

  

  Manual download
• Biospin Yeast Plasmid DNA Extraction Kit MORE
  Product Details：

  Kit Components

  Cat#	BSC01S1G	BSC01M1G
  Components	50Tests	100Tests
  RS Buffer	30.0ml	60.0ml
  Resuspension Buffer	12.5ml	25ml
  Lysis Buffer	12.5ml	25ml
  Neutralization Buffer	17.5ml	35ml
  PW Buffer	25ml	50ml
  Wash Buffer	13.0ml (add 52ml ethanol before use)	26.0ml (add 104ml ethanol before use)
  Snailase	260mg×2 （2-8℃）	260mg×4（2-8℃）
  RNase A	50ul  （2-8℃）	100ul （2-8℃）
  Elution Buffer	10.0ml	20.0ml
  Spin columns	50	100
  Handbook	1copy	1copy

  Introduction

      The Kit provides a fast, simple, and cost-effective yeast plasmid miniprep method for routine  molecular biology laboratory applications. Yeast plasmid DNA can be purified from 3–5ml of overnight cultures of yeast. The DNA isolated by this Kit is ready for downstream applications such as transformation, sequencing, PCR/Real-time PCR and other downstream experiments.

  Product Features：

  Storage and Transportation

  • The kit should be stored at room temperature(15~25℃), but the Snailase and the RNase A should be stored at 2~8℃,the Snailase solution should be stored at -20℃.The kit can be stored for up to 12 months by this method. After addition of RNase A, Resuspension Buffer should be stored 2~8℃.
  • The kit can be transported at room temperature.

  Materials to Be Supplied by the User

  * Sterile 1.5ml micro centrifuge tubes     * Absolute ethanol     *β-mercaptoethanol

  Important notes

  1. Please use 1.3 ml RS Buffer to oscillation dissolve each tube snailase, then hold - 20 ℃.
  2. The β-mercaptoethanol should be added into the RS Buffer (10ul/ml) before use. This mixture is  good at room temperature for 1 week.
  3. The RNase A should be all added into the Resuspension Buffer before use, mix and store at 2-8℃.
  4. Add ethanol (as the volume is marked on bottle label) to Wash Buffer then mix well.
  5. If the Lysis Buffer and Neutralization Buffer precipitated, it should be redissolved by warming to 37°C. Please not vortex Lysis Buffer acutely.
  6. Please close the lid immediately after using Lysis Buffer so as to avoid acidification.
  7. The Kit can extract high-quality yeast plasmid DNA from 3-5ml yeast culture overnight cultured.

  Experimental data:

  DNA Analysis

  • Absorbance analysis

  Get some plasmid DNA，diluted in a advisable factor with elution buffer. Survey the OD260, OD280 and OD320

  Expressions：concentration（μg/ml）＝50×OD260×dilution fact

  Target： 2.0≥OD260-320/ OD280-320≥1.8

  Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

  • Agarose Gel Analysis
    0.8～1％ Agarose gel

  Example 1：Plasmid DNA electrophoresis

  DL15, 000                                    DL2, 000

  

  Example 2：Enzymatic reactions analysis

       DL2, 000                                                                                                         DL15, 000

  

  Manual download
• Biospin Whole Blood Genomic DNA Midi Extraction Kit MORE
  Product Details：

  Kit Components

  Cat#	BSC06S1B
  Components	10Tests
  PK solution	1.5ml
  Balance Buffer	10ml
  5×RCL Buffer	60ml
  NS Buffer	20ml
  LysisB Buffer	20ml
  WB1 Buffer	17.2ml （add22.8ml ethanol before use）
  Wash Buffer	21ml （add 49ml ethanol before use）
  Elution Buffer	20.0ml
  MidiSpin column	10
  Handbook	1 copy

  Introduction

      The kit provides a very simple, fast and effective technique for the isolation of pure high– molecular-weight genomic DNA in 10ml whole blood which was treated with anticoagulants such as citrate, heparin, EDTA. Based on the remarkable selectivity on genome DNA of Midispin membrane, the simple purification procedure involves only a few steps and allows isolation of high yields of pure genomic   DNA within 2hours. No any expensive equipment are required, using of toxic or hazardous reagents, such as phenol or chloroform is completely avoided. In general, 150～250μg genomic DNA can be acquired from 10ml blood using the Kit. The pure DNA can be applied extensively in PCR/Real-time PCR、sequencing、 Southern blot、mutant analysis、SNP, and so on.

  Product Features：

  Storage

  • The PK solution and 5×RCL Buffer must be stored at 2～8℃, other components in the kit may be deposited at room temperature.
  •  All components, when stored properly, can keep stable for 18months.

  Apparatus and Materials should Be Supplied by the User

  *  50ml sterile Micro-centrifuge tubes                 * 10µl/100µl/1000µl tips

  *  Micro-centrifuge capable of 5,000×g               * Absolute ethanol (>99%)

  *  Vortex mixer                                                 * warm bath

  Before Starting：

  (1)     According to the label on the bottle, add ethanol to WB1 buffer and Wash buffer.

  (2)       Add 4 volumes of deionized water to 1 volume of 5×RCL Buffer.

  (3)      Add 1ml Balance Buffer to each MidiSpin column, centrifuge at 5,000 x g for 3 min. Discard flow-through.

  Experimental data:

  Analysis DNA

  • Absorbance analysis

  Take some DNA，dilute it into an appropriate concentration with elution buffer. Measure it at OD260，OD280 and OD320.

  Equation：concentration（μg/ml）＝50×OD260×dilution multiple

  target：2.0≥OD260-320/ OD280-320≥1.7

  Notice: 1.0≥OD260≥0.1, the result of ratio is reliable.

  •  Agarose Gel Analysis

  0.8～1％ Agarose gel

  

  Manual download
• Biospin Whole Blood Genomic DNA Maxi Extraction Kit MORE
  Product Details：

  Kit Components

  Cat#	BSC06S1C
  Components	10Tests
  PK solution	5ml
  Balance Buffer	20ml
  LysisB Buffer	70ml×2
  WB1 Buffer	25.8ml （add 34.2ml ethanol before use）
  Wash Buffer	64ml （add 96ml ethanol before use）
  Elution Buffer	20.0ml
  MaxiSpin column	10 tubes
  Handbook	1 copy

  Introduction

      The kit provides a very simple, fast and effective technique for the isolation of pure high– molecular-weight genomic DNA in 10ml whole blood based on the remarkable selectivity on genome DNA of Maxispin membrane, the simple purification procedure involves only a few steps and allows isolation of high yields of pure genomic DNA within 2hours. No any expensive equipment are required, using of  toxic or  hazardous  reagents,  such  as  phenol  or  chloroform  is  completely  avoided.  In  general, 150～250μg genomic DNA can be acquired from 10ml blood using the Kit. The pure DNA can be applied extensively in PCR/Real-time PCR、sequencing、Southern blot、mutant analysis、SNP, and so on.

  Product Features：

  Storage

  • The PK solution must be stored at 2～8℃, other components in the kit may be deposited at room temperature.
  • All components, when stored properly, can keep stable for 18months.

  Apparatus and Materials should Be Supplied by the User

  *  50ml sterile Micro-centrifuge tubes       * 10µl/100µl/1000µl tips

  *  Micro-centrifuge capable of 5,000×g     * Absolute ethanol (>99%)

   *  Vortex mixer                                      * warm bath

  Before Starting：

  1. According to the label on the bottle, add ethanol to WB1 buffer and Wash buffer.
  2. Add 2 ml Balance Buffer to each MaxiSpin column, centrifuge at 5,000 x g for 3 min. Discard flow-through.

  Note：Each elution typically yields 60%of DNA bound to the column. To obtain DNA at higher concentrations, please suck the liquid collected in the collection tube again to the column. Incubate at room temperature for 2 minutes. Centrifuge at 4,000×g for 3 minutes. The DNA in the collection tube is ready for further analysis. If the isolated DNA sample is not going to be tested on the same day, freeze it at -20℃.

  Experimental data:

  Analysis DNA

  •  Absorbance analysis

  Take some DNA，dilute it into an appropriate concentration with elution buffer. Measure it at OD260，OD280 and OD320.

  Equation：concentration（μg/ml）＝50×OD260×dilution multiple

  target：2.0≥OD260-320/ OD280-320≥1.7

  Notice: 1.0≥OD260≥0.1, the result of ratio is reliable.

  • Agarose Gel Analysis 

  0.8～1％ Agarose gel

  Example 1：2ml whole blood

  
  

  Example 2：5ml Blood clots

  
  

  Manual download
• Biospin Virus RNA Extraction Kit MORE
  Product Details：

  Kit Components

  Cat#	BSC62S1	BSC62M1
  Components	50Tests	100Tests
  RLysis Buffer	15ml	30ml
  RD Buffer	17.5ml	35ml
  DNase Stop Buffer	5.0ml (add 6.5ml ethanol before use)	10.0ml (add 13ml ethanol before use)
  Wash Buffer	20ml (add 30ml ethanol before use)	40ml (add 60ml ethanol before use)
  RElution Buffer	10ml	20ml
  Spin Columns	50	100
  Handbook	1copy	1copy

  Introduction

      The kit is a ready-to-use reagent for the isolation of Virus RNA from different type’s sample. Add RLysis Buffer to the processed sample and adding alcohol will bind RNA to spin column. Then RNA can be easily isolated through several washing and eluting steps.

      The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-throughput. The pure RNA can be applied extensively in Northern blot, blotting hybridization, in vitro translation, RNase protect assay, RT-PCR/Real-time RT-PCR analysis, construction cDNA library etc.

  Product Features：

  Storage and transportation

  • The kit has demonstrated stability of 24 months when stored at room temperature.
  • The kit can be transported at room temperature.

  Technical Information

  Sample	Amount
  Animal tissue	≤30mg
  Culture cells	≤1×108
  White blood cells	≤from 5ml whole blood
  For liquid sample	≤100μl

  Apparatus and materials to be prepared by the user

  *  Sterile 1.5ml microcentrifuge tubes            * 10µl/100µl/1000µl tips

  *  Microcentrifuge capable of 14,000rpm       * 70℃ Water bath      * Vortex mixer

  *  Liquid nitrogen (or ice bath)     * Optional: β-Me        * PBS          *Trypsination

  *  Red Blood Cells Lysis Buffer（Cat#BSA06M1）         * Absolute alcohol

  Important note

  • RD Buffer may be precipitated at low temperature, the heated at 37 ℃ for a few minutes, to restore the clarification.
  • DNase Stop Buffer Add the alcohol as the volume marked on bottle label and mix well.
  • Wash Buffer Add the alcohol as the volume marked on bottle label and mix well.

  Experimental data:

  Analysis RNA

  Example：HCV Real-time RT-PCR  （Sample HCV concentration：copies/ml）

  

  

  

  Manual download
• Biospin Total RNA Extraction Kit MORE
  Product Details：

  Kit Components

  Cat#	BSC63S1	BSC63M1
  Components	50Tests	100Tests
  RLysis Buffer	8.75ml	17.5ml
  RD Buffer	17.5ml	35ml
  DNase Buffer	2ml	4ml
  MnCl2	450ul	900ul
  DNase I	50ul(stored at -20℃)	100ul(stored at -20℃)
  DNase Stop Buffer	5 ml (add 6.5 ml ethanol before use)	10 ml (add 13 ml ethanol before use)
  Wash Buffer	32 ml (add 48ml ethanol before use)	32 ml (add 48ml ethanol before use)×2
  RElution Buffer	10ml	20ml
  Spin columns	50	100
  Handbook	1 copy	1 copy

  Introduction

      The kit is a ready-to-use reagent for the isolation of total RNA from animal tissues, cells, bacteria and others (plant tissues are not recommended). Add RLysis Buffer to the processed sample.RD buffer will remove the protein, then adding alcohol will bind RNA to spin column. The DNA will be destroyed by the DNase I reaction. Then RNA can be easily isolated through several washing and eluting steps.

      The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-throughput. The pure RNA can be applied extensively in Northern blot, blotting hybridization, in vitro translation,RNase protect assay, RT-PCR/Real time RT-PCR analysis, construction cDNA library etc.

  Product Features：

  Storage and transportation

  • The kit has demonstrated stability of 18 months when the DNase I should be stored at -20 ℃, others at room temperature.

  • The kit can be transported at room temperature.

  Apparatus and materials to be prepared by the user

  *  Sterile 1.5ml centrifuge tubes                       * 10µl/100µl/1000µl tips

  *  Microcentrifuge capable of 14,000rpm           * Absolute alcohol

  *  Vortex mixer                                              * β- mercaptoethanol

   Important note

  • DNase Stop Buffer: Add the alcohol as the volume marked on bottle label and mix well.
  • Wash Buffer: Add the alcohol as the volume marked on bottle label and mix well.

  Experimental data:

  Analysis RNA

  • Absorbance analysis

  Get some RNA，diluted in a advisable factor with elution buffer. Survey the OD260，OD280 and OD320.

  Expressions：concentration（μg/ml）＝40×OD260×dilution fact

  Target：2.0≥OD260-320/ OD280-320≥1.7

  Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

  • Agarose Gel Analysis

  

  Manual download
• Biospin SwabGen DNA Extraction Kit MORE
  Product Details：

   Kit Components

   

  Cat#	BSC28S1	BSC28M1
  Components	50Tests	100Tests
  PK solution	500ul	1000ml
  LysisB Buffer	30ml	60ml
  WB1 Buffer	12ml (add 17ml ethanol before use)	24ml (add 34ml ethanol before use)
  Wash Buffer	18ml (add 42ml ethanol before use)	36ml (add 84ml ethanol before use)
  Elution Buffer	10 ml	20 ml
  Spin column	50 tubes	100 tubes
  Handbook	1copy	1copy

  Introduction

      The kit provides a very simple, fast and effective technique for the isolation of pure high– molecular-weight genomic DNA in Oral swabs. Based on the remarkable selectivity on genome DNA of Biospin membrane, the simple purification procedure involves only a few steps and allows isolation of high yields of pure genomic DNA within 30 minutes. No any expensive equipments are required, using of  toxic or  hazardous  reagents,  such  as  phenol  or  chloroform  is  completely  avoided.  In  general,   0.5～3.5μg genomic DNA can be acquired using the Kit. The pure DNA can be applied extensively in PCR/Real-time PCR、sequencing、Southern blot、mutant analysis、SNP, and so on.

  Product Features：

  Storage and transportation

  • The PK solution must be stored at 2～8℃, other components in the kit may be deposited at room temperature.
  • All components, when stored properly, can keep stable for 18months.
  • The kit can be transported at room temperature.

  Apparatus and materials to be prepared by the user

  *  1.5ml and 2.0ml sterile micro-centrifuge tubes   * 10µl/100µl/1000µl tips

  *  Micro-centrifuge capable of 12,000×g                * Absolute ethanol (>99%)

  *  Vortex mixer                                                  * Warm bath or metal bath

  Important note

  Add the ethanol (as the volume marked on bottle label) to WB1 buffer and wash buffer and mix them well.

  Experimental data:

  Analysis DNA

  •  Absorbance analysis

   Take some DNA，dilute it into an appropriate concentration with elution buffer.

   Measure it at OD260，OD280 and OD320.

  Equation：concentration（μg/ml）＝50×OD260×dilution multiple

  target：2.0≥OD260-320/ OD280-320≥1.7

  Notice: 1.0≥OD260≥0.1, the result of ratio is reliable.

  • Agarose Gel Analysis 0.8～1％ Agarose gel

   SYBR Green Real-time PCR

  

  Manual download
• Biospin Polyacrylamide Gel DNA Extraction Kit MORE
  Product Details：

  Kit Components

  Cat#	BSC15S1	BSC15M1
  Components	50Tests	100Tests
  PE Buffer	10 ml	20 ml
  PEB Buffer	20 ml	40 ml
  PWash Buffer	18.9ml	37.8ml
  Elution Buffer	10ml	20ml
  Spin Column	50	100
  Handbook	1copy	1copy

  Introduction

      The kit provides a simple, rapid and effective method for purification of DNA fragments from polyacrylamide gel. The simple purification procedure is based on the remarkable selectivity of Biospin membrane. There need few steps to finish extraction without expensive equipment, as completely avoids using toxic and hazardous reagents such as phenol and chloroform. Purified DNA can be used directly for kinds of downstream molecular biological experiments such as cloning, sequencing, PCR, restriction enzyme digestion, and so on.

  Product Features：

  Storage and transportation

  • The kit should be stored dry at room temperature（15～25℃）, The kit can be stored for up to 18 months if all components are kept properly.
  • The kit can be transported at room temperature.

  Technical Information

  Method	Column volume	DNA size range	Electrophoresis buffer	Incubate temperature	Elution recovery
  Spin column	750µl	100bp～10kb	TAE/TBE buffer	65℃	≥99％

  Apparatus and Materials to Be Supplied by the User

  * Sterile 1.5 or 2.0 ml microcentrifuge tubes        * 10µl/100µl/1000µl tips

  *  Absolute ethanol    * Microcentrifuge capable of 14,000g * Vortex mixer   * Water bath or cooling & heating block

  Preparation

   

  1. This product cannot be used with PAGE with urea.
  2. Add ethanol as per the volume marked on label of bottle into Wash Buffer and then mix well.
  3. If the gel slice is very big, please increase the dosage of PE Buffer properly but on more than 200μl, thus to guarantee that the gel slice is immerged completely.
  4. The suitable volume of elution buffer for the kit is 50µl. The dosage of elution buffer can be adjusted according to practical experimental situation.
  5. Please wash the fixed gel by water or 10mM Tris-HCl (pH7.0) for 2 or 3 times if the gel is fixed by acidic reagent such as acetic acid.
• Biospin Plasmid DNA Maxi Extraction Kit MORE
  Product Details：

  Kit Components

  Cat#	BSC01S1C	BSC01M1C
  Components	10Tests	25Tests
  Balance Buffer	20ml	50ml
  Resuspension Buffer	100ml	250ml
  Lysis Buffer	100ml	250ml
  Neutralization Buffer	120ml	100ml
  Washing Buffer	50mlх2 （add 75ml ethanol before use）	104mlх2 （add 156ml ethanol before use）
  Elution Buffer	60ml	150ml
  RNase A	1 tube	1 tube
  Max Spin column	10 tubes	25 tubes
  Handbook	1 copy	1 copy

  Introduction

       This kit allows the high efficient binding of DNA to our spin matrix while proteins and  other  impurities are removed by wash buffer. Nucleic acids are easily eluted with sterile water or Elution Buffer. This kit is designed for fast and efficient purification of plasmid DNA from 150 to 200 ml of E. coli culture. The maxi column has a plasmid DNA binding capacity of 1 mg.

      The purified plasmid DNA is ready for downstream applications such as transfection of robust cells such as HEK293, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations.

  Product Features：

  Storage and transportation

  •  The kit has demonstrated stability of 18 months when the RNase A should be stored at 2-8℃,others at room temperature.
  • The kit can be transported at room temperature.
  •  Resuspension Buffer should be stored at 2°C-8℃ once RNase A is added.

  Apparatus and materials to be prepared by the user

   *  50ml centrifuge tubes                            * 10µl/100µl/1000µl tips

   *  Centrifuge capable of 14,000g               * Absolute alcohol

  Important note

  1）Spin down RNase A vial briefly. Add the RNase A solution to Resuspension Buffer and mix well before use. Resuspension Buffer should be stored at 2°C-8℃ once RNase A is added.

  2）Wash Buffer: Add the alcohol as the volume marked on bottle label and mix well.

  3）Lysis Buffer precipitates below room temperature. It is critical to warm up the buffer at 50°C to dissolve the precipitates before use.

  4）Neutralization Buffer may form precipitates below 10°C, warm up at 37°C to dissolve the precipitates before use.

  5）Carry out all centrifugations at room temperature.

  6) Add 2ml Balance Buffer to each MaxiSpin column, incubate at room temperature for 2 mins,centrifuge at 5,000 x g for 3 min. Discard flow-through. It can improve the yield of DNA significantly.